主要科学研究领域：

生物电分析化学。

主持项目：

1. 国家自然科学基金，禽白血病病变组织中microRNA异常表达的电化学研究。

2. 山东省自然科学基金，MicroRNA的电化学检测及对植物发育过程中植物激素调控机理研究。

3. 作物生物学国家重点实验室开放课题，MicroRNAs在细胞分裂素信号传导途径中作用机理的光电化学研究。

4. 中国博士后基金一等资助，蛋白激酶活性的光电化学分析及抑制剂筛选。

5. 中国博士后科学基金特别资助，肝癌细胞基因组DNA羟甲基化的光电化学检测。

参加项目：

1. 国家自然科学基金，功能树状高分子组装电化学基因探针-NASBA联用技术及快速检测禽流感病毒的研究（No.20775044）。

2. 国家自然科学基金，超灵敏电化学基因传感器的构筑及对环境激素致病机理的研究（No. 21075078）。

3. 国家自然科学基金，土壤中硫丹的微生物强化降解机制与生态毒性去除效应（No. 41071164）。

4. 国家自然科学基金，一氧化氮调控桃果实山梨醇脱氢酶活性的分子机理（No. 30901160）。

奖励：

1. 2011年，“食品污染物的高灵敏检测、高效控制及毒理研究”获山东高等学校优秀科研成果奖——自然科学类二等奖，第二位。

2. 2013年，“肿瘤细胞生物指示物和环境致癌物高灵敏检测的研究”获2013年山东高等学校优秀科研成果奖——自然科学类二等奖，第二位。

3. 2013年，获“第十一届泰安市青年科技奖”。

4. 2015年，“MicroRNA-21对禽白血病指示作用的电化学研究”获山东高等学校优秀科研成果奖——自然科学类三等奖，第一位。

5. 2015年，“DNA甲基转移酶活性的电化学分析及抑制剂筛选”获山东高等学校优秀科研成果奖——自然科学类三等奖，第二位。

部分代表性论文（*为通讯作者）：

[1]Zhiqing Yang, Wenjing Jiang, Fei Liu, Yunlei Zhou, Huanshun Yin*, Shiyun Ai*, A novel electrochemical immunosensor for the quantitative detection of 5-hydroxymethylcytosine in genomic DNA of breast cancer tissue, Chemical Communications, 51 (2015) 14671-14673.

[2]Mo Wang, Zhengliang Fu, Bingchen Li, Yunlei Zhou, Huanshun Yin*, Shiyun Ai*, One-step, ultrasensitive and electrochemical assay of microRNAs based on T7 exonuclease assisted cyclic enzymatic amplification, Analytical Chemistry, 86 (2014) 5606-5610.

[3]Mo Wang, Zhenning Xu, Lijian Chen, Huanshun Yin*, Shiyun Ai*, An electrochemical immunosensing platform for DNA methyltransferase activity analysis and inhibitor screening, Analytical Chemistry, 84 (2012) 9072-9078.

[4]Huanshun Yin, Zhiqing Yang, Bingchen Li, Yunlei Zhou*, Shiyun Ai*, Electrochemical biosensor for DNA demethylase detection based on demethylation triggered endonuclease BstUI and Exonuclease III digestion, Biosensors and Bioelectronics, 66 (2015) 266-270.Huanshun Yin,

[5]Mo Wang, Bingchen Li, Zhiqing Yang, Yunlei Zhou*, Shiyun Ai*, A sensitive electrochemical biosensor for detection of protein kinase A activity and inhibitors based on Phos-tag and enzymatic signal amplification, Biosensors and Bioelectronics, 63 (2015) 26-32.

[6]Huanshun Yin, Bing Sun, Linfeng Dong, Bingchen Li, Yunlei Zhou*, Shiyun Ai*, A signalon photoelectrochemical biosensor for assay of protein kinase activity and its inhibitor based on graphite-like carbon nitride, Phos-tag and alkaline phosphatase, Biosensors and Bioelectronics, 64 (2015) 462-468.

[7]Zhiqing Yang, Fengrui Wang, Mo Wang, Huanshun Yin*, Shiyun Ai*, A novel signal-on strategy for M.SssI methyltransfease activity analysis and inhibitor screening based on photoelectrochemical immunosensor, Biosensors and Bioelectronics, 66 (2015) 109-114.

[8]Yunlei Zhou, Mo Wang, Zhenning Xu, Cailing Ni, Huanshun Yin*, Shiyun Ai*, Investigation of the effect of phytohormone on the expression of microRNA-159a in Arabidopsis thalianaseedlings based on mimic enzyme catalysis systematic electrochemical biosensor, Biosensors and Bioelectronics, 54 (2014) 244-250.

[9]Yunlei Zhou, Bingchen Li, Mo Wang, Zhiqing Yang, Huanshun Yin*, Shiyun Ai*, Enzyme-based electrochemical biosensor for sensitive detection of DNA demethylation and the activity of DNA demethylase, Analytica Chimica Acta, 840 (2014) 28-32.

[10]Mo Wang, Huanshun Yin*, Nannan Shen, Zhenning Xu, Bing Sun, Shiyun Ai*, Signal-on photoelectrochemical biosensor for microRNA detection based on Bi₂S₃ nanorods and enzymatic amplification, Biosensors and Bioelectronics, 53 (2014) 232-237.

[11]Bing Sun, Lijian Chen, Yan Xu, Min Liu, Huanshun Yin*, Shiyun Ai*, Ultrasensitive photoelectrochemical immunoassay of indole-3-acetic acid based on the MPA modified CdS/RGO nanocomposites decorated ITO electrode, Biosensors and Bioelectronics, 51 (2014) 164-169.

[12]Huanshun Yin, Yunlei Zhou, Zhenning Xu, Mo Wang, Shiyun Ai, Ultrasensitive electrochemical immunoassay for DNA methyltransferase activity and inhibitor screening based on methyl binding domain protein of MeCP2 and enzymatic signal amplification, Biosensors and Bioelectronics, 49 (2013) 39-45.

[13]Zhenning Xu, Huanshun Yin*, Yunxiang Han, Yunlei Zhou, Shiyun Ai*, DNA-based hybridization chain reaction amplification for assaying the effect of environmental phenolic hormone on DNA methyltransferase activity, Analytica Chimica Acta, 829 (2014) 9-14.

[14]Huanshun Yin, Yunlei Zhou, Zhenning Xu, Lijian Chen, Di Zhang, Shiyun Ai*, An electrochemical assay for DNA methylation, methyltransferase activity and inhibitor screening based on methyl binding domain protein, Biosensors and Bioelectronics, 41 (2013) 492-497.

[15] Huanshun Yin, Zhenning Xu, Yunlei Zhou, Mo Wang, Shiyun Ai, An ultrasensitive electrochemical immunosensor platform with double signal amplification for indole-3-acetic acid determinations in plant seeds, Analyst, 138 (2013) 1851-1857.

注：招收生物传感和功能材料制备方向的研究生。

通信地址：山东省泰安市岱宗大街61号山东农业大学化学与材料科学学院

邮政编码：271018

电子邮箱：yinhs@sdau.edu.cn

联系电话：0538-8247660（实验室）